Board Thread:Speculation House/@comment-28792888-20140620221642/@comment-4010415-20160216001806

WC-83 wrote: It's from the latest episode. 4th to last column, second image, just above her lower eyelid. You can tell from the slight blue tint in places, same as any other tear animation, only smaller. The one from Battle of Beacon was definatly just white shine (but never know).

EDIT: This one

http://rwby.wikia.com/wiki/File:V3_12_00120.png Yeah, no, those are definitely not tears. Those are the shine on her irises. You can especially tell because the one in the eye on our right is in an odd place but placed exactly the same as the other one in regards to her iris.